Anthelmintic Efficacy Testing Step by Step


1. Find a group of egg count positive horses sharing the same pastures.


a. At least 5 horses are recommended, but more will provide better precision.


b. If less than 5 horses are available, proceed with the test but exercise more caution with interpreting the results. c. A treatment check can be carried out for a single horse.


2. Count as many parasite eggs as possible pre-treatment. a. 40 counted eggs/horse or more. b. Some techniques will count more eggs than others.


c. Counting more slides from each horse will also increase the number of eggs counted.


3. Administer the anthelmintic at the labelled dosage. 4. Collect new fecal samples at 14 days post treatment.


5. Determine fecal egg counts using the same technique and number of slides as for the pre-treatment samples.


6. Calculate the 90% confidence intervals for the percent reduction for the group by use of online calculation software https://www.fecrt.com/.


a. You can also calculate the mean percent reduction for the group or a single horse using the formula: ((total eggs counted pre – total eggs counted post)/ total eggs counted pre) X 100%.


7. Interpret your results according to the thresholds and guidance provided in the report generated from fecrt.com and herein.


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