HOW TO INCORPORATE ASSISTED REPRODUCTIVE TECHNOLOGIES Acknowledgments
Declaration of Ethics The Authors have adhered to the Principles of Veter- inary Medical Ethics of the AVMA.
Conflict of Interest The Authors have no conflicts of interest.
References
1. Choi YH, Love LB, Varner DD, et al. Holding immature equine oocytes in the absence of meiotic inhibitors: Effect on germinal vesicle chromatin and blastocyst development after
2. Salustri A, Yanagishita M, Underhill CB, et al. Localization and synthesis of hyaluronic acid in the cumulus cells and mural granulosa cells of the preovulatory follicle. Dev Biol 1992;151:541–551.
3. Mahadevan MM, Trounson AO. Removal of the cumulus oo- phorus from the human oocyte for in vitro fertilization. Fertil Steril 1985;43:263–267.
4. Choi YH, Varner DD, Love CC, et al. Production of live foals via intracytoplasmic injection of lyophilized sperm and sperm extract in the horse. Reproduction 2011;142:529–538.
5. Saunders CM, Larman MG, Parrington J, et al. PLC£, a sperm-specific trigger of CA2
development. Development 2002;129:3533–3544. oscillations in eggs and embryo