LAMENESS: DIAGNOSTIC ANALGESIA, MEDICATIONS, AND PHYSIOLOGIC EFFECTS
of time. No major impact of the isolation method on proliferation, migration, and differentiation behav- ior of the MSCs could be detected. Interestingly, compared with MSCs isolated by explant technique, a significantly higher expression level of the tendon marker scleraxis was found in MSCs isolated by collagenase digestion.
4. Discussion
Differences in cell characteristics caused by isola- tion methods could make enzymatically isolated
MSCs advantageous for certain clinical applications. Furthermore, higher obtainable cell numbers could enable an efficient practice of enzymatically isolated MSCs.
Acknowledgment
This work was funded by the Mehl-Muelhens Foundation.
