54


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KELLEY


TABLE 1 The minimum dilution ratio (semen: extender) for cooled- shipped semen based on the raw sperm concentration of semen and the progressive motility cut- off values for centrifugation by concentration of the ejaculate.


Raw concentration range (×10 6 sperm/mL)


<125


125– 250 250– 300 300– 350 350– 400 400– 450 450– 500 500– 550 550– 600 600– 650 650– 700 700– 750


Dilution ratio (semen: Extender)


Centrifuge 1:4 1:5 1:6 1:7 1:8 1:9


1:10 1:11 1:12 1:13 1:14


Progressive motility Cut- off (%) a


Extended concentration (×10 6 sperm/mL)


Specific sperm concentrations of raw semen


125 130 135 140 145 150 155 160 165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 252 258 264 270 276 282 288 294 300 301 308 315 322 329 336 343 350 352 360 368 376 384 392 400 405 414 423 432 441 450 460 470 480 490 500 506 517 528 539 550 552 564 576 588 600 611 624 637 650 658 672 686 700 705 720 735 750 36


67 25 65 26 62 27 60 28 58 29 56 30 54 31 53 32 51 33 49 34 48 35 47 36 45 44 43 37 38 39 42 40 41 40 41 42 39 43 38 44 37 45 37 46 47 35 48 34 49 Note : If the raw semen sperm concentration is <125 × 10 6 then centrifugation is needed, for other concentrations the minimum dilution ratio is listed in the adjacent column. In the table are specific


concentrations for each range and the corresponding minimum progressive motility needed to avoid having an insemination dose with a volume > 60 mL. a If the progressive motility is less than the cut- off value, centrifugation is recommended to avoid having a large insemination volume (>60 mL). The progressive motility cut- off values are calculated for a breeding dose with a total number of progressively motile sperm of ≥1 × 10 9 and a volume of 60 mL.


34 50


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