one abnormal endocrine test) (n21), and clinical PPID (hirsutism and two or more abnormal endocrine tests) (n 25). Ethylenediaminetetraacetic acid (EDTA) plasma adrenocorticotropic hormone (ACTH) was measured by Cornell.
3. Results and Discussion
Within non-PPID, subclinical PPID, and clinical PPID groups, repeated measures (RM) ANOVAs demonstrated basal, T10, and T30 ACTH values all varied significantly between months (P .001). Two-way RM ANOVA demonstrated basal, T10, and T30 ACTH values all varied significantly with both PPID status and season (P.001). Youden indices were used to determine seasonal and monthly cutoff
values for basal, T10, and T30 ACTH concentra- tions, which should be considered when diagnosing PPID.
Acknowledgments
Declaration of Ethics The Authors have adhered to the Principles of the Veterinary Medical Ethics of the AVMA.
Conflict of Interest The Authors declare no conflicts of interest.
Funding Source This project was funded by Morris Animal Foundation.
