Received: 6 May 2022 | Accepted: 15 August 2023 DOI: 10.1111/eve.13888
OR IG INA L A R T I C L E
The therapeutic effect of stem cell- derived exosomes in the treatment of chronic endometritis as assessed by histopathological, Doppler and hormonal expression in Arabian mares
Elshymaa A. Abdelnaby 1,2
1 Department of Clinical Sciences, College of Veterinary Medicine , King Faisal University , Alahsa , Saudi Arabia 2 Theriogenology Department, Faculty of Veterinary Medicine , Cairo University , Giza , Egypt 3 Hormones Department, Medical Research and Clinical Studies Institute , National Research Centre , Giza , Egypt 4 Faculty of Veterinary Medicine , Cairo University , Giza , Egypt 5 Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine , Cairo University , Giza , Egypt
Correspondence : Elshymaa A. Abdelnaby Email:
elshymaa.ahmed@cu.edu.eg and
elshymaaahmed@yahoo.com
| Ahmed N. Abdallah 3 | Islam M. Anwar 4 | Omar S. El- Tookhy 5 | Ashraf A. Shamaa 5
Summary Background : Bone- derived exosomes are considered nano- sized extracellular vesicles secreted by the bone marrow stromal cells. Objectives : This study used exosomes derived from bone marrow mesenchymal stem cells to treat chronic endometritis in Arabian mares with follow- up alterations in haemo- dynamics, hormonal and histopathological expressions. Methods and study design : Fourteen ( n = 14) Arabian mares (aged 13– 15 years old) were equally divided into the control group and an endometritis group (chronic type). Exosomes were infused twice 21 days apart at the first and second ovulations. Follow- up was done ultrasonographically, and samples were taken for histopathology. Blood samples were taken to estimate hormonal levels. Results : Uterine biopsy samples before treatment showed a marked degree of degenera- tion, while the second exosomal treatment showed regression of fibrous tissue and res- toration of healthy endometrial glands with normal epithelium. Uterine body and horns thickness/mm were decreased after treatment at day 0 of the 3rd ovulation compared to day −1 before treatment, the uterine body- coloured area and total uterine coloured areas/pixels were elevated ( p < 0.05) at day 0 of the 3rd ovulation compared to day −1 before treatment. Uterine artery blood parameters (pulsatility index, resistance index, peak systolic and end- diastolic points) did not change before, during and after treatment, but the blood flow rate was elevated ( p < 0.05) on day 0 of the 3rd ovulation compared to day −1 before treatment. Oestradiol level was decreased ( p < 0.05), while progesterone level was increased ( p < 0.05) on day 0 of the 3rd ovulation compared to day −1 before treatment. Conclusion : Two intrauterine administrations of exosomes restored the normal endome- trial appearance after chronic endometritis resulting in the elevation of the conception rate in infertile mares.
K E YWORDS horse , Arabian mare , Doppler , endometritis , exosomes
Equine Vet Educ. 2024;36:347–356.
[Correction added on 14 September 2023, after first online publication: The affiliation of Ahmed N. Abdallah was corrected in this version.]
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