Based on the results, ovariectomy under general non-barbiturate anesthesia before euthanasia is the recommended practice to avoid the potential effects of pentobarbital on oocytes. A future study to evaluate the effect of the detected concentration of pentobarbital on oocyte developmental compe- tence is warranted.
Acknowledgments
Declaration of Ethics The Authors have adhered to the Principles of Veteri- naryMedical Ethics of theAVMA.
Conflict of Interest The Authors have no conflicts of interest.
Footnote aEuthasol®, Dechra Veterinary Products, Overland Park, KS